Vitamins, Minerals and Supplements

Introduction


Minerals


RDA Values for Vitamins and Minerals


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Water Soluble Vitamins Fat Soluble Vitamins
Thiamine (B1)
B1 Deficiency and Disease

Riboflavin (B2)
B2 Deficiency and Disease

Niacin (B3)
B3 Deficiency and Disease
Pantothenic Acid (B5)
Pyridoxal, Pyridoxamine, Pyridoxine (B6)
Biotin
Cobalamin (B12)
B12 Deficiency and Disease

Folic Acid
Folate Deficiency and Disease

Ascorbic Acid
Vitamin A
Gene Control by Vitamin A
Role of Vitamin A in Vision
Additional Roles of Vitamin A
Clinical Significances of Vitamin A

Vitamin D
Clinical Significances of Vitamin D

Vitamin E
Clinical Significances of Vitamin E

Vitamin K
Clinical Significance of Vitamin K

Alpha-Lipoic Acid, LA


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Introduction to Vitamins and Minerals

Vitamins are organic molecules that function in a wide variety of capacities within the body. The most prominent function of the vitamins is to serve as cofactors (co-enzymes) for enzymatic reactions. The distinguishing feature of the vitamins is that they generally cannot be synthesized by mammalian cells and, therefore, must be supplied in the diet. The vitamins are of two distinct types, water soluble and fat soluble. For more information on the food products that are good sources of the individual vitamins visit the Supplement Science page.

 

 

 

 

 

 

 

 

 

 

The minerals that are considered of dietary significance are those that are necessary to support biochemical reactions by serving both functional and structural roles as well as those serving as electrolytes. The use of the term dietary mineral is considered archaic since the intent of the term "mineral" is to describe ions not actual minerals. There are both quantity elements required by the body and trace elements. The quantity elements are sodium, magnesium, phosphorous, sulfur, chlorine, potassium and calcium. The essential trace elements are manganese, iron, cobalt, nickel, copper, zinc, selenium, molybdenum, and iodine. Additional trace elements (although not considered essential) are boron, chromium, fluoride, and silicon.

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Thiamine (Thiamin)

Structure of thiamin

Thiamine structure

Thiamine (also written thiamin) is also known as vitamin B1 . Thiamin is derived from a substituted pyrimidine and a thiazole which are coupled by a methylene bridge. Thiamine is rapidly converted to its active form, thiamin pyrophosphate, TPP, in the brain and liver by a specific enzyme, thiamine diphosphotransferase.

Structure of thiamin pyrophosphate

Thiamine pyrophosphate

TPP is necessary as a cofactor for three critical dehydrogenases. These enzymes are pyruvate dehydrogenase (PDH) and α-ketoglutarate dehydrogenase (αKGDH; associated with the TCA cycle), and branched-chain ketoacid dehydrogenase (BCKD) necessary for metabolism of the branched-chain amino acids, leucine, isoleucine, and valine. In addition to these three dehydrogenases TPP is a required co-factor for the transketolase catalyzed reactions of the pentose phosphate pathway. A deficiency in thiamine intake leads to a severely reduced capacity of cells to generate energy and to carry out reductive biosynthetic reactions as well as to synthesize nucleotides as a result of its role in each of these enzymes.

The dietary requirement for thiamine is proportional to the caloric intake of the diet and ranges from 1.0–1.5 mg/day for normal adults. If the carbohydrate content of the diet is excessive then an increase in  thiamine intake will be required.

The richest sources of vitamin B1 include yeasts and animal liver. Additional sources include whole-grain cereals, rye and whole-wheat flour, navy beans, kidney beans, wheat germ, as well as pork and fish.

Food source

Thiamine content (mg)

Yeast, brewer's, 2 tbls 2.3
Pork chop, lean, 3.5 oz 0.9
Ham, lean, 3.5 oz 0.7
Catfish, 3.5 oz cooked 0.4
Bagel, 2 oz enriched 0.4
Milk, soy, 1 cup 0.4
Beans, baked, 1 cup 0.34
Oatmeal, 1 cup cooked 0.26
Rice, white, cooked, 1 cup 0.26
Green peas, ½ cup cooked 0.23
Potato, one medium baked 0.22
Orange juice, 1 cup 0.20
Black beans, ½ cup cooked 0.21
Navy beans, ½ cup cooked 0.19
Soy nuts, ½ cup 0.20
Cashews, ½ cup 0.15
Peanuts, ½ cup 0.10
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Clinical Significances of Thiamine Deficiency

The earliest symptoms of thiamine deficiency include constipation, appetite suppression, and nausea. Progressive deficiency will lead to mental depression, peripheral neuropathy and fatigue. Chronic thiamine deficiency leads to more severe neurological symptoms including ataxia, mental confusion and loss of eye coordination (nystagmus). A highly diagnostic physical test of thiamine deficiency is vertical nystagmus. Vertical nystagmus is characterized by spontaneous upbeating or downbeating of the eyeball. There are numerous causes or horizontal nystagmus but vertical is only seen due to the CNS damage associated with thiamine deficiency or with phencyclidine (PCP) intoxication. Additional clinical symptoms of prolonged thiamine deficiency are related to cardiovascular and musculature defects.

Dietary thiamine deficiency is known as beri beri, is most often the result of a diet that is carbohydrate rich and thiamine deficient. An additional thiamine deficiency related syndrome is known as Wernicke syndrome which is most often associated with chronic alcohol consumption. This disease is most commonly found in chronic alcoholics due to the fact that alcohol impairs thiamine uptake from the small intestine as well as the fact that these individuals generally have poor dietetic lifestyles. Wernicke syndrome is also referred to as dry beri beri. Prolonged dietary deficiency in thiamine leads to wet beri beri. The wet form of the disease is the result the cardiac involvement in the deficiency. At this stage in the deficiency all four chambers of the heart enlarge due to loss of energy generation and fluid retention resulting in what is called dilated cardiomyopathy. The result of the enlarged chambers is that they can't fill completely resulting in systolic failure. Systole relates to the force associated with cardiac contraction expelling blood to arteries. Blood pumped from the left ventricle enters the aorta and is delivered to the body, whereas blood pumped from the right ventricle is sent to the lungs.

When thiamine deficiency manifests with CNS involvement it is called Korsakoff encephalopathy (or Korsakoff psychosis) and is also commonly referred to as Wernicke-Korsakoff syndrome (WKS). WKS is characterized by acute encephalopathy progressing to chronic impairment of short-term memory. Thiamine supplementation can reverse the symptoms of beri beri and Wernicke syndrome, however, the consequences of severe deficiency (WKS) are irreversible. The confabulation of Korsakoff psychosis is due to destruction of the mammillary bodies in the brain. The mammillary bodies are composed of two small round structures at the underside of the brain that are part of the limbic system, specifically they are part of the Papez circuit. This circuit is also called the hippocampal-mammillo-thalamo-cortical pathway. The consequence of destruction of the mammillary bodies is retrograde amnesia.

Persons afflicted with an inherited form of Wernicke-Korsakoff syndrome appear to have an inborn error of metabolism that is clinically important only when the diet is inadequate in thiamine. These individuals are thought to harbor an abnormality in the enzyme, transketolase. Although a variant transketolase enzyme has been proposed to be associated with Wernicke-Korsakoff syndrome, no mutations have been found in the gene encoding this enzyme when cloned from patients exhibiting the syndrome.

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Riboflavin

Structure of riboflavin

Riboflavin structure

Riboflavin is also known as vitamin B2. Riboflavin is the precursor for the coenzymes, flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). Synthesis of these two cofactors occurs in a two step process. FMN is synthesized from riboflavin via the ATP-dependent enzyme riboflavin kinase (RFK). RFK introduces a phosphate group onto the terminal hydroxyl of riboflavin. FMN is then converted to FAD via the attachment of AMP (derived from ATP) though the action of FAD pyrophosphorylase which is also known as FMN adenylyltransferase (FMNAT).

The enzymes that require FMN or FAD as cofactors are termed flavoproteins. Several flavoproteins also contain metal ions and are termed metalloflavoproteins. Both classes of enzymes are involved in a wide range of redox reactions, e.g. succinate dehydrogenase and xanthine oxidase. During the course of the enzymatic reactions involving the flavoproteins the reduced forms of FMN and FAD are formed, FMNH2 and FADH2, respectively. The hydrogens of FADH2 are on nitrogens 1 and 5 as indicated in the figure. The normal daily requirement for riboflavin is 1.2–1.7 mg/day for normal adults.

Structure of FAD

Structure of FAD

Riboflavin is found in dairy products, lean meats, poultry, fish, grains, broccoli, turnip greens, asparagus, spinach, and enriched food products.

Food source

Riboflavin content (mg)

Beef liver, 3.5oz cooked 4.14
Mackerel, 3.5 oz canned 0.54
Pork, loin, 3 oz cooked 0.24
Hamburger, lean, 3.5 oz 0.21
Chicken, dark, 3 oz cooked 0.19
Steamed clams, 3.5 oz 0.43
Yogurt, low-fat, 1 cup 0.37
Egg, cooked 0.25
Cheese, cottage, ½ cup 0.21
Milk, nonfat, 1 cup 0.34
Pasta, 1 cup cooked 0.23
Bagel, plain 0.22
Spinach, ½ cup cooked 0.16
Wheat germ, raw, 2 tbls 0.12
Soy nuts, ½ cup 0.65
Almonds, ½ cup 0.78
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Clinical Significances of Flavin Deficiency

Riboflavin deficiencies are rare in the United States due to the presence of adequate amounts of the vitamin in eggs, milk, meat and cereals. Riboflavin deficiency is often seen in chronic alcoholics due to their poor dietetic habits.

Symptoms associated with riboflavin deficiency include itching and burning eyes, angular stomatitis and cheilosis (cracks and sores in the mouth and lips), bloodshot eyes, glossitis (inflammation of the tongue leading to purplish discoloration), seborrhea (dandruff, flaking skin on scalp and face), trembling, sluggishness, and photophobia (excessive light sensitivity). Riboflavin decomposes when exposed to visible light. This characteristic can lead to riboflavin deficiencies in newborns treated for hyperbilirubinemia by phototherapy.

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Niacin

Structure of nicotinamide Structure of nicotinic acid

Nicotinamide

Nicotinic Acid

Niacin (nicotinic acid and nicotinamide) is also known as vitamin B3. Both nicotinic acid and nicotinamide can serve as the dietary source of vitamin B3. Niacin is required for the synthesis of the active forms of vitamin B3, nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+). Both NAD+ and NADP+ function as cofactors for numerous dehydrogenases, e.g., lactate dehydrogenase and malate dehydrogenase.

Structure of NAD

The –OH phosphorylated in NADP+ is indicated by the red arrow. NADH is shown in the box insert.

Structure of NAD+

Niacin is not a true vitamin in the strictest definition since it can be derived from the amino acid tryptophan. However, the ability to utilize tryptophan for niacin synthesis is inefficient (60 mg of tryptophan are required to synthesize 1 mg of niacin). Also, synthesis of niacin from tryptophan requires vitamins B1, B2 and B6 which would be limiting in themselves on a marginal diet. The recommended daily requirement for niacin is 13–19 niacin equivalents (NE) per day for a normal adult. One NE is equivalent to 1 mg of free niacin).

Niacin is found in liver, meat, peanuts and other nuts, and whole grains. In addition, foods that are rich in protein, with exception of tryptophan-poor grains, can satisfy some of the demand for niacin.

Food source

Niacin content (mg)

Beef liver, 3.5oz cooked 14.4
Chicken, white meat, cooked 13.4
Tuna, canned in water, 3 oz 11.8
Salmon, 3.5 oz cooked 8.0
Ground beef, 3.5 oz cooked 5.3
Peanuts, ½ cup 10.5
Almonds, ½ cup 1.4
Potato, baked with skin 3.3
Mushrooms, raw, ½ cup 1.7
Barley, ½ cup cooked 1.6
Corn, yellow, ½ cup 1.3
Lentils, ½ cup cooked 1.4
Sweet potatoes, ½ cooked 1.2
Carrot, raw, medium 0.7
Peach, raw, medium 0.9
Mango, 1 medium 1.5
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Clinical Significances of Niacin and Nicotinic Acid

A diet deficient in niacin (as well as tryptophan) leads to glossitis of the tongue (inflammation of the tongue leading to purplish discoloration), dermatitis, weight loss, diarrhea, depression and dementia. The severe symptoms, depression, dermatitis and diarrhea, are associated with the condition known as pellagra. Several physiological conditions (e.g. Hartnup disorder and malignant carcinoid syndrome) as well as certain drug therapies (e.g. isoniazid) can lead to niacin deficiency. In Hartnup disease tryptophan absorption is impaired and in malignant carcinoid syndrome tryptophan metabolism is altered resulting in excess serotonin synthesis. Isoniazid (the hydrazide derivative of isonicotinic acid) is a primary drug for chemotherapy of tuberculosis.

Nicotinic acid (but not nicotinamide) when administered in pharmacological doses of 2–4 g/day lowers plasma cholesterol levels and has been shown to be a useful therapeutic for hypercholesterolemia. The major action of nicotinic acid in this capacity is a reduction in fatty acid mobilization from adipose tissue. Although nicotinic acid therapy lowers blood cholesterol it also causes a depletion of glycogen stores and fat reserves in skeletal and cardiac muscle. Additionally, there is an elevation in blood glucose and uric acid production. For these reasons nicotinic acid therapy is not recommended for diabetics or persons who suffer from gout.

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Pantothenic Acid

Structure of pantothenic acid

Pantothenic Acid

Pantothenic acid is also known as vitamin B5. Pantothenic acid is formed from β-alanine and pantoic acid. In the synthesis of coenzyme A from pantothenate there are five reaction steps. Pantothenate is phosphorylated on the hydroxyl group via the action of pantothenate kinase. The reactive sulfhydryl group is added from cysteine via the action of phosphopantothenoylcysteine synthetase. Through three more reactions the molecule is decarboxylated and then the ADP from ATP is added forming the fully functional coenzyme A shown in the Figure below.

Pantothenate is required for synthesis of coenzyme A, CoA and is a component of the acyl carrier protein (ACP) domain of fatty acid synthase. Pantothenate is, therefore, required for the metabolism of carbohydrate via the TCA cycle and all fats and proteins. At least 70 enzymes have been identified as requiring CoA or ACP derivatives for their function.

Food source

Vitamin B5 content (mg)

Beef liver, 3.5 oz 5.3
Poultry, dark meat, 3.5 oz 1.3
Poultry, white meat, 3.5 oz 1.0
Salmon, 3.5 oz cooked 1.4
Low fat yogurt, 1 cup 1.5
Milk, nonfat, 1 cup 0.8
Bleu cheese, 1 oz 0.49
Cottage cheese, ½ cup 0.27
Corn, cooked, ½ cup 0.72
Potato, baked, one 0.7
Sweet potato, ½ cup 0.68
Broccoli, boiled, ½ cup 0.4
Wheat germ, raw, ¼ cup 1.2
Mushrooms, cooked, ½ cup 0.84
Peanuts, ½ cup 0.9
Avocado half 1.0
Sunflower seeds, ¼ cup 2.3
Dates, 10 0.65 
Papaya, ½ cup 0.33 
Strawberries, 1 cup 0.25 
Orange juice, 1 cup 0.24 

Deficiency of pantothenic acid is extremely rare due to its widespread distribution in whole grain cereals, legumes and meat. Symptoms specific to pantothenate deficiency are difficult to assess since they are subtle and resemble those of other B vitamin deficiencies. These symptoms include painful and burning feet, skin abnormalities, retarded growth, dizzy spells, digestive disturbances, vomiting, restlessness, stomach stress, and muscle cramps.

Structure of coenzyme A (CoA)

Coenzyme A

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Vitamin B6

Structure of pyridoxine Structure of pyridoxal Structure of pyridoxamine

Pyridoxine

Pyridoxal

Pyridoxamine

Pyridoxal, pyridoxamine and pyridoxine are collectively known as vitamin B6. All three compounds are efficiently converted to the biologically active form of vitamin B6, pyridoxal phosphate (PLP). This conversion is catalyzed by the ATP requiring enzyme, pyridoxal kinase. Pyridoxal kinase requires zinc for full activity thus making it a metaloenzyme.

Structure of pyridoxal phosphate

Pyridoxal Phosphate

Pyridoxal phosphate functions as a cofactor in enzymes involved in transamination reactions required for the synthesis and catabolism of the amino acids as well as in glycogenolysis as a cofactor for glycogen phosphorylase and as a co-factor for the synthesis of the inhibitory neurotransmitter γ-aminobutyric acid (GABA). Pyridoxal phosphate is also a required co-factor for the synthesis of heme at the initial and regulated step in this pathway catalyzed by δ-aminolevulinic acid synthase (ALAS). The requirement for vitamin B6 in the diet is proportional to the level of protein consumption ranging from 1.4–2.0 mg/day for a normal adult. During pregnancy and lactation the requirement for vitamin B6 increases approximately 0.6 mg/day.

Deficiencies of vitamin B6 are rare and usually are related to an overall deficiency of all the B-complex vitamins. Isoniazid (see niacin deficiencies above) and penicillamine (used to treat rheumatoid arthritis and cystinurias) are two drugs that complex with pyridoxal and PLP resulting in a deficiency in this vitamin. Deficiencies in pyridoxal kinase result in reduced synthesis of PLP and are associated with seizure disorders related to a reduction in the synthesis of GABA. Other symptoms that may appear with deficiency in vitamin B6 include nervousness, insomnia, skin eruptions, loss of muscular control, anemia, mouth disorders, muscular weakness, dermatitis, arm and leg cramps, loss of hair, slow learning, and water retention.

Food source

Vitamin B6 content (mg)

Beef liver, 3.5 oz 1.4
Turkey, light meat, 3.5 oz 0.5
Chicken, light meat, 3.5 oz 0.63
Salmon, 3.5 oz cooked 0.65
Halibut, baked, 3.5 oz 0.4
Potatoes, 1 cup 0.48
Sweet potatoes, ½ cup 0.3
Oatmeal, 1 cup cooked 0.74
Rice, brown, cooked, 1 cup 0.28
Brussels sprouts, ½ cup 0.23
Lentils, ½ cup cooked 0.18
Carrots, ½ cup cooked 0.18
Peanuts, ½ cup 0.18
Sunflower seeds, ¼ cup 0.26
Avacado, 1 Haas 0.48
Mango 0.28
Watermelon, 1 cup 0.22
Cantelope, 1 cup 0.18 
Prunes, 10 dried 0.22 
Blackstrap molasses, 2 tbls 0.29 
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Biotin

Structure of biotin

Biotin

Biotin is the cofactor required of enzymes that are involved in carboxylation reactions, e.g. acetyl-CoA carboxylase and pyruvate carboxylase. Biotin is found in numerous foods and also is synthesized by intestinal bacteria and as such deficiencies of the vitamin are rare. Deficiencies are generally seen only after long antibiotic therapies which deplete the intestinal fauna or following excessive consumption of raw eggs. The latter is due to the affinity of the egg white protein, avidin, for biotin preventing intestinal absorption of the biotin. Symptoms that may appear if biotin is deficient are extreme exhaustion, drowsiness, muscle pain, loss of appetite, depression, and grayish skin color.

Some of the richest sources of biotin are swiss chard, tomatoes, romaine lettuce, and carrots. Additional sources include onions, cabbage, cucumber, cauliflower, mushrooms, peanuts, almonds, walnuts, oat meal, bananas, raspberries, strawberries, soy, egg yolk, and cow and goat milk.

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Cobalamin

Cobalamin is more commonly known as vitamin B12. Vitamin B12 is composed of a complex tetrapyrrol ring structure (corrin ring) and a cobalt ion in the center. Vitamin B12 is synthesized exclusively by microorganisms and is found in the liver of animals bound to protein as methycobalamin or 5'-deoxyadenosylcobalamin, as well as in milk and soybeans. The vitamin must be released from these binding proteins in order to be active and this release occurs in the stomach by gastric acids. After being released, cobalamin is bound to an endogenous protein called haptocorrin (also known transcobalamin I, TCN1). Within the small intestine pancreatic trypsin hydrolyzes the haptocorrin-cobalamin complexes allowing binding of cobalamin to another protein called intrinsic factor (IF; also called gastric intrinsic factor, GIF) within the alkaline pH of the intestines. Haptocorrin and intrinsic factor are two of the three vitamin B12-binding proteins produced in humans. The third is transcobalamin II (TCN2) which is a plasma globulin that is the primary transport protein for vitamin B12 in the blood.

Intrinsic factor is a 50-kDa glycoprotein produced by the parietal cells of the stomach. The secretion of intrinsic factor is stimulated by all three of the mechanisms that lead to gastrric acid secretion: histamine, acetylcholine, and gastrin. Vitamin B12 bound to intrinsic factor can then bind to a receptor complex called cubilin present in the apical membranes of ileal enterocytes. Cubilin is also found in epithelial cells and has been shown to be involved in apoA-I-mediated binding of HDL. Upon intrinsic factor-vitamin B12-binding to cubilin, the complex in endocytosed. The intrinsic factor in the endocytosed complexes  is degraded by lysosomal hydrolases. Cobalamin then binds to transcobalamin II and this complex is transported out of the enterocytes into the portal circulation.

There are only two clinically significant reactions in the body that require vitamin B12 as a cofactor. During the catabolism of fatty acids with an odd number of carbon atoms and the amino acids valine, isoleucine and threonine the resultant propionyl-CoA is converted to succinyl-CoA for oxidation in the TCA cycle. One of the enzymes in this pathway, methylmalonyl-CoA mutase, requires vitamin B12 as a cofactor in the conversion of methylmalonyl-CoA to succinyl-CoA. The 5'-deoxyadenosine derivative of cobalamin is required for this reaction.

The second reaction requiring vitamin B12 catalyzes the conversion of homocysteine to methionine and is catalyzed by methionine synthase. This reaction results in the transfer of the methyl group from N5-methyltetrahydrofolate to hydroxycobalamin generating tetrahydrofolate (THF) and methylcobalamin during the process of the conversion.

Structure of cobalamin, vitamin B12

Vitamin B12 (cobalamin)

The recommended daily intake (RDA) of vitamin B12 for adults is 2.4 micrograms (μg). Vitamin B12 is found primarily in animal products. Due to the lack of sufficient vitamin B12 in plant foods it is added to breakfast cereals and this serves as a good source of the vitamin for vegetarians. Two plant sources that are useful for obtaining vitamin B12 are alfalfa and comfrey (also written comfry). However, to ensure adequate intake vegans should use a vitamin B12 supplement that contains at least 5–10μg due to the low absorption rate of the vitamin in supplement form.

Food source

Vitamin B12 content (mcg: μg)

Beef liver, 3.5 oz 48
Rainbow trout, wild, 3.0 oz 5.4
Salmon, 3.0 oz 4.9
Beef, 3.0 oz 2.4
Tuna, white, 3.0 oz 1.0
Yogurt, plain, 1 cup 1.4
Fortified cereal, 100% RDA 6.0
Milk, 1 cup 0.9
Swiss cheese, 1 oz 0.9
Egg, 1 whole 0.6
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Clinical Significances of B12 Deficiency

The liver can store up to six years worth of vitamin B12, hence deficiencies in this vitamin are rare. Pernicious anemia is a megaloblastic anemia resulting from vitamin B12 deficiency that develops as a result a lack of intrinsic factor in the stomach leading to malabsorption of the vitamin. The anemia results from impaired DNA synthesis due to a block in purine and thymidine biosynthesis. The block in nucleotide biosynthesis is a consequence of the effect of vitamin B12 on folate metabolism. When vitamin B12 is deficient essentially all of the folate becomes trapped as the N5-methylTHF derivative as a result of the loss of functional methionine synthase (also called homocysteine methyltransferase). This trapping prevents the synthesis of other THF derivatives required for the purine and thymidine nucleotide biosynthesis pathways.

Neurological complications also are associated with vitamin B12 deficiency and result from a progressive demyelination of nerve cells. The demyelination is thought to result from the increase in methylmalonyl-CoA that result from vitamin B12 deficiency. Methylmalonyl-CoA is a competitive inhibitor of malonyl-CoA in fatty acid biosynthesis as well as being able to substitute for malonyl-CoA in any fatty acid biosynthesis that may occur. Since the myelin sheath is in continual flux the methylmalonyl-CoA-induced inhibition of fatty acid synthesis results in the eventual destruction of the sheath. The incorporation methylmalonyl-CoA into fatty acid biosynthesis results in branched-chain fatty acids being produced that may severely alter the architecture of the normal membrane structure of nerve cells.

Deficiencies in B12 can also lead to elevations in the level of circulating homocysteine. Elevated levels of homocysteine are known to lead to cardiovascular dysfunction. Due to its high reactivity to proteins, homocysteine is almost always bound to proteins, thus thiolating them leading to their degradation. Homocysteine also binds to albumin and hemoglobin in the blood. The detrimental effects of homocysteine are thought to be due to its' binding to lysyl oxidase, an enzyme responsible for proper maturation of the extracellular matrix proteins collagen and elastin. Production of defective collagen and elastin has a negative impact on arteries, bone and skin and the effects on arteries are believed to be the underlying cause for cardiac dysfunction associated with elevated serum homocysteine. In individuals with homocysteine levels above ≈12μM there is an increased risk of thrombosis and cardiovascular disease.

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Folic Acid

Structure of folic acid

positions 7 and 8 carry hydrogens in dihydrofolate (DHF)

positions 5–8 carry hydrogens in tetrahydrofolate (THF)

Folic Acid

Folic acid is a conjugated molecule consisting of a pteridine ring structure linked to para-aminobenzoic acid (PABA) that forms pteroic acid. Folic acid itself is then generated through the conjugation of glutamic acid residues to pteroic acid. Folic acid is obtained primarily from yeasts and leafy vegetables as well as animal liver. Animal cannot synthesize PABA nor attach glutamate residues to pteroic acid, thus, requiring folate intake in the diet.

When stored in the liver or ingested folic acid exists in a polyglutamate form. Intestinal mucosal cells remove some of the glutamate residues through the action of the lysosomal enzyme, conjugase. The removal of glutamate residues makes folate less negatively charged (from the polyglutamic acids) and therefore more capable of passing through the basal lamina membrane of the epithelial cells of the intestine and into the bloodstream. Folic acid is reduced within cells (principally the liver where it is stored) to tetrahydrofolate (THF also H4folate) through the action of dihydrofolate reductase (DHFR), an NADPH-requiring enzyme.

The function of THF derivatives is to carry and transfer various forms of one carbon units during biosynthetic reactions. The one carbon units are either methyl, methylene, methenyl, formyl or formimino groups.

Active center of tetrahydrofolate (THF)

Note that the N5 position is the site of attachment of methyl groups, the N10 the site for attachment of formyl and formimino groups and that both N5 and N10 bridge the methylene and methenyl groups.

Active center of tetrahydrofolate (THF).

These one carbon transfer reactions are required in the biosynthesis of serine, methionine, glycine, choline and the purine nucleotides and dTMP.

The ability to acquire choline and amino acids from the diet and to salvage the purine nucleotides makes the role of N5,N10-methylene-THF in dTMP synthesis the most metabolically significant function for this vitamin. The role of vitamin B12 and N5-methyl-THF in the conversion of homocysteine to methionine also can have a significant impact on the ability of cells to regenerate needed THF.

The recommended daily allowance for folic acid is 400 micrograms (μg) for both men and women except that pregnant women should increase their intake to at least 600 μg/day. The best sources for folic acid are cereals that have been fortified with 100% of the RDA of folic acid. Beef liver (3 oz) contains 45% of the RDA of folic acid. Excellent vegetarian sources for folic acid are baked beans, raw spinach, asparagus, green peas, broccoli, lentils, turnip greens, egg noodles, avocado, peanuts, lettuce, wheat germ, tomato juice and orange juice.

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Clinical Significances of Folate Deficiency

Folate deficiency results in complications nearly identical to those described for vitamin B12 deficiency. The most pronounced effect of folate deficiency on cellular processes is upon DNA synthesis. This is due to an impairment in dTMP synthesis which leads to cell cycle arrest in S-phase of rapidly proliferating cells, in particular hematopoietic cells. The result is megaloblastic anemia as for vitamin B12 deficiency. The inability to synthesize DNA during erythrocyte maturation leads to abnormally large erythrocytes termed macrocytic anemia.

Folate deficiencies are rare due to the adequate presence of folate in food. Poor dietary habits as those of chronic alcoholics can lead to folate deficiency. The predominant causes of folate deficiency in non-alcoholics are impaired absorption or metabolism or an increased demand for the vitamin. The predominant condition requiring an increase in the daily intake of folate is pregnancy. This is due to an increased number of rapidly proliferating cells present in the blood. The need for folate will nearly double by the third trimester of pregnancy. Certain drugs such as anticonvulsants and oral contraceptives can impair the absorption of folate. Anticonvulsants also increase the rate of folate metabolism.

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Ascorbic Acid

Structure of ascorbic acid

Ascorbic Acid

Ascorbic acid is more commonly known as vitamin C. Ascorbic acid is derived from glucose via the uronic acid pathway. The enzyme L-gulonolactone oxidase responsible for the conversion of gulonolactone to ascorbic acid is absent in primates making ascorbic acid required in the diet.

The active form of vitamin C is ascorbic acid itself. The main function of ascorbate is as a reducing agent in a number of different reactions. Ascorbate is the cofactor for Cu+–dependent monooxygenases and Fe2+–dependent dioxygenases. Ascorbate has the potential to reduce cytochromes a and c of the respiratory chain as well as molecular oxygen. The most important reaction requiring ascorbate as a cofactor is the hydroxylation of proline residues in collagen. Vitamin C is, therefore, required for the maintenance of normal connective tissue as well as for wound healing since synthesis of connective tissue is the first event in wound tissue remodeling. Vitamin C also is necessary for bone remodeling due to the presence of collagen in the organic matrix of bones.

Ascorbic acid also serves as a reducing agent and an antioxidant. When functioning as an antioxidant ascorbic acid itself becomes oxidized to semidehydroascorbate and then dehydroascorbate. Semidehydroascorbate is reconverted to ascorbate in the cytosol by cytochrome b5 reductase and thioredoxin reductase in reactions involving NADH and NADPH, respectively. Dehydroascorbate, the fully oxidized form of vitamin C, is reduced spontaneously by glutathione, as well as enzymatically in reactions using glutathione or NADPH.

Several other metabolic reactions require vitamin C as a cofactor. These include the catabolism of tyrosine and the synthesis of epinephrine from tyrosine and the synthesis of the bile acids. It is also believed that vitamin C is involved in the process of steroidogenesis since the adrenal cortex contains high levels of vitamin C which are depleted upon adrenocorticotropic hormone (ACTH) stimulation of the gland.

Deficiency in vitamin C leads to the disease scurvy due to the role of the vitamin in the post-translational modification of collagens. Scurvy is characterized by easily bruised skin, muscle fatigue, soft swollen gums, decreased wound healing and hemorrhaging, osteoporosis, and anemia. Vitamin C is readily absorbed and so the primary cause of vitamin C deficiency is poor diet and/or an increased requirement. The primary physiological state leading to an increased requirement for vitamin C is severe stress (or trauma). This is due to a rapid depletion in the adrenal stores of the vitamin. The reason for the decrease in adrenal vitamin C levels is unclear but may be due either to redistribution of the vitamin to areas that need it or an overall increased utilization.

Inefficient intake of vitamin C has also been associated with a number of conditions, such as high blood pressure, gallbladder disease, stroke, some cancers, and atherosclerosis (plaque in blood vessels that can lead to heart attack and stroke). Sufficient vitamin C in the diet may help reduce the risk of developing some of these conditions, however, the evidence that taking vitamin C supplements will help or prevent any of these conditions is still lacking.

The amount of vitamin C that is recommended to consume each day (the RDA) depends upon the age and sex of the individual. Infants less than 1 year old should get 50 milligrams (mg) per day. children 1–3 years old need 15mg, 4–8 years old need 25mg, and 9–13 years old need 45mg. Adolescent girls should get 65mg per day and adolescent boys should get 75mg per day. Adult males need 90mg per day and adult women should get 75mg per day. Women who are breastfeeding should increase their intake to at least 120mg per day. Individuals who smoke should increase their daily intake by at least 35mg since smoking depletes vitamin C levels. The recommended daily intake of vitamin C to prevent conditions such as the cardiovascular disorders indicated above is reported to be between 500mg and 1000mg.

Excellent sources of vitamin C are fruits and vegetables such as oranges, watermelon, papaya, grapefruit, cantaloupe, strawberries, raspberries, blueberries, cranberries, pineapple, kiwi, mango, green peppers, broccoli, turnip greens, spinach, red and green peppers, canned and fresh tomatoes, potatoes, Brussels sprouts, cauliflower, and cabbage. Citrus juices or juices fortified with vitamin C are also excellent sources of the vitamin.

Vitamin C is sensitive to light, air, and heat, so the most vitamin C is available in fruits and vegetables that are eaten raw or lightly cooked. Natural or synthetic vitamin C can be found in a variety of forms. Tablets, capsules, and chewables are probably the most popular forms, but vitamin C also comes in powdered crystalline, effervescent, and liquid forms. An esterified form of vitamin C is also available, which may be easier on the stomach for those who are prone to heartburn. The best way to take vitamin C supplements is 2–3 times per day, with meals, depending on the dosage.

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Vitamin A

Vitamin A consists of three biologically active molecules, retinol, retinal (retinaldehyde) and retinoic acid.

Structure of all-trans-retinal Structure of 11-cis-retinal

All-trans-retinal

11-cis-retinal

 
Structure of retinol Structure of retinoic acid

Retinol

Retinoic Acid

Each of these compounds are derived from the plant precursor molecule, β-carotene (a member of a family of molecules known as carotenoids). Beta-carotene, which consists of two molecules of retinal linked at their aldehyde ends, is also referred to as the provitamin form of vitamin A.

Ingested β-carotene is cleaved in the lumen of the intestine by β-carotene dioxygenase to yield retinal. Retinal is reduced to retinol by retinaldehyde reductase, an NADPH requiring enzyme within the intestines. Retinol is esterified to palmitic acid and delivered to the blood via chylomicrons. The uptake of chylomicron remnants by the liver results in delivery of retinol to this organ for storage as a lipid ester within lipocytes. Transport of retinol from the liver to extrahepatic tissues occurs by binding of hydrolyzed retinol to aporetinol binding protein (RBP). the retinol-RBP complex is then transported to the cell surface within the Golgi and secreted. Within extrahepatic tissues retinol is bound to cellular retinol binding protein (CRBP). Plasma transport of retinoic acid is accomplished by binding to albumin.

Vitamin A is found in dark green and yellow vegetables and yellow fruits, such as broccoli, spinach, turnip greens, carrots, squash, sweet potatoes, pumpkin, cantaloupe, and apricots, and in animal sources such as liver, milk, butter, cheese, and whole eggs. When determining the amount of vitamin A to consumw each day the RDA is expressed as retinol activity equivalents (RAE).


Food source

Vitamin A content (mcg: μg RAE)

Organ meats, cooked, 3 oz 1490–9126
Sweetpotato with peel (1 medium), baked 1096
Carrots, cooked from fresh, ½ cup 671
Spinach, cooked from frozen, ½ cup 573
Kale, cooked from frozen, ½ cup 478
Beet greens, cooked, ½ cup 276
Dandelion greens, cooked, ½ cup 260
Cantaloupe, raw, ¼ medium melon 233
Mustard greens, cooked, ½ cup 221
Red sweet pepper, cooked, ½ cup 186
Chinese cabbage, cooked, ½ cup 180
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Gene Control Exerted by Retinoic Acid

Within cells retinoic acid (as well as the related compound 9-cis-retinoic acid) bind to specific receptor proteins that are members of the nuclear receptors family of hormone receptors. Following binding, the receptor-retinoic acid complex interacts with specific sequences in several genes involved in growth and differentiation and affects expression of these genes. In this capacity retinoic acid is considered a hormone of the steroid/thyroid hormone superfamily of proteins. The retinoic acid receptors are abbreviated RAR and the related retinoid X receptors (RXRs) bind 9-cis-retinoic acid. Several genes, whose patterns of expression are altered by retinoic acid, are involved in the earliest processes of embryogenesis including the differentiation of the three germ layers, organogenesis and limb development. To date, there are at least 130 genes that have been shown to be directly regulated (either positive or negative) via interaction with RAR or RXR.

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Vision and the Role of Vitamin A

Photoreception in the eye is the function of two specialized neuron types located in the retina; the rod and cone cells. The rod cells are utilized only for seeing at night During the day rod cells continuously release the excitatory neurotransmitter glutamate. The released glutamate binds to the synaptic membranes of specialized cells called bipolar cells. The glutamate receptor on bipolar cells is the mGluR6 metabotropic receptor. For more information on the glutamate receptors go to the Biochemistry of Nerve Transmission page. Activation of bipolar cells induces them to release the inhibitory neurotransmitter, GABA. Release of GABA, thus leads to inhibition of the optic nerve.

Both rod and cone cells contain a photoreceptor pigment in their membranes. The photosensitive compound of most mammalian eyes is a protein called opsin to which is covalently coupled an aldehyde of vitamin A. The opsin of rod cells is called scotopsin. The photoreceptor of rod cells is specifically called rhodopsin or visual purple. This compound is a complex between scotopsin and the 11-cis-retinal (also called 11-cis-retinene) form of vitamin A. Rhodopsin is a G-protein coupled receptor (GPCR) embedded in the membrane of the discs inside the outer segment of rod cells. Coupling of 11-cis-retinal occurs at three of the transmembrane domains of rhodopsin. Intracellularly, rhodopsin is coupled to a specific G-protein called transducin. The specific Gα-subunit of transduction is identified as Gαt.

role of vitamin A in the function of visual signaling via rod cells

Vitamin A and visual signaling in rod cells: When the rhodopsin is exposed to light it is bleached resulting in the conversion of 11-cis-retinal to all-trans-retinal. The conformational change in retinal converts rhodopsin to metarhodopsin II. The change to metarhodopsin II in turn activates the associated G-protein, tranducin. Activated transducin exhibits an increased GTP-binding by the α-subunit. Binding of GTP releases the α-subunit from the inhibitory βγ subunits. The GTP-activated α-subunit in turn activates an associated cGMP phosphodiesterase (PDE); an enzyme that hydrolyzes cyclic-GMP (cGMP) to GMP. This PDE is specifically identified as PDE6B. Cyclic GMP is required to maintain the Na+ channels of the rod cell plasma membrane in the open conformation. The drop in cGMP concentration results in complete closure of the Na+ channels. This sodium channel is a member of a family of cyclic nucleotide-gated (CNG) ion channels. The rod cell sodium channel is a heterotetramer consisting of two α and two β subunits. These subunits are encoded by the CNGA1 and CNGB1 genes, respectively. When the sodium channel closes it leads to hyperpolarization of the rod cell. The hyperpolarization causes the closure on calcium channels in the plasma membrane and since calcium ions are required for the release of glutamate from pre-synaptic vesicles the release of glutamate is inhibited. The loss of glutamate release results less activation of the mGLuR6 receptor on bipolar cells with the consequences that these cells become depolarized and no longer release the inhibitory neurotransmitter, GABA. The result is that the optic nerve become disinhibited in the dark so that we can see with the limited light available at night.

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Additional Role of Retinol

Retinol also functions in the synthesis of certain glycoproteins and mucopolysaccharides necessary for mucous production and normal growth regulation. This is accomplished by phosphorylation of retinol to retinyl phosphate which then functions similarly to dolichol phosphate.

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Clinical Significances of Vitamin A Deficiency

Vitamin A is stored in the liver and deficiency of the vitamin occurs only after prolonged lack of dietary intake. The earliest symptoms of vitamin A deficiency are night blindness. Additional early symptoms include follicular hyperkeratinosis, increased susceptibility to infection and cancer and anemia equivalent to iron deficient anemia. Prolonged lack of vitamin A leads to deterioration of the eye tissue through progressive keratinization of the cornea, a condition known as xerophthalmia.

The increased risk of cancer in vitamin deficiency is thought to be the result of a depletion in β-carotene. Beta-carotene is a very effective antioxidant and is suspected to reduce the risk of cancers known to be initiated by the production of free radicals. Of particular interest is the potential benefit of increased β-carotene intake to reduce the risk of lung cancer in smokers. However, caution needs to be taken when increasing the intake of any of the lipid soluble vitamins. Excess accumulation of vitamin A in the liver can lead to toxicity which manifests as bone pain, hepatosplenomegaly, nausea and diarrhea.

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Vitamin D

Vitamin D is a steroid hormone that functions to regulate specific gene expression following interaction with its intracellular receptor (VDR: vitamin D receptor). The VDR is a member of the steroid and thyroid hormone superfamily of nuclear receptors. The biologically active form of the hormone is 1,25-dihydroxy vitamin D3 (1,25-(OH)2D3, also called 1,25-dihydroxycholecalciferol or calcitriol). Calcitriol functions primarily to regulate calcium and phosphorous homeostasis.

Structure of ergosterol Structure of 7-dehydrocholesterol

Ergosterol

7-Dehydrocholesterol

 
Structure of vitamine D2 Structure of vitamin D3

Vitamin D2

Vitamin D3

Active calcitriol is derived from ergosterol (produced in plants) and from 7-dehydrocholesterol. 7-dehydrocholesterol is an intermediate in the synthesis of cholesterol that accumulates in the skin. Upon exposure to ultraviolet (uv) light from the sun and following thermal isomerization, 7-dehydrocholesterol is non-enzymatically converted to pre-vitamin D3 which then enters the bloodstream and is taken up by the liver where it undergoes the first of two activating hydroxylation reactions. Ergocalciferol (vitamin D2) is formed by uv irradiation of ergosterol.

Vitamin D2 and D3 are processed to D2-calcitriol and D3-calcitriol, respectively, by the same enzymatic pathways in the body. Cholecalciferol (or ergocalciferol) are absorbed from the intestine and transported to the liver bound to a specific vitamin D-binding protein. In the liver cholecalciferol is hydroxylated at the 25 position by a specific D3-25-hydroxylase generating 25-hydroxy-D3 [25-(OH)D3] which is the major circulating form of vitamin D. Conversion of 25-(OH)D3 to its biologically active form, calcitriol, occurs through the activity of a specific D3-1-hydroxylase present in the proximal convoluted tubules of the kidneys, and in bone and placenta. 25-(OH)D3 can also be hydroxylated at the 24 position by a specific D3-24-hydroxylase in the kidneys, intestine, placenta and cartilage.

Structure of 25-hydroxyvitamin D3 Structure of 1,25-dihydroxyvitamin D3

25-hydroxyvitamin D3

1,25-dihydroxyvitamin D3

Calcitriol functions in concert with parathyroid hormone (PTH) and calcitonin to regulate serum calcium and phosphorous levels. PTH is released in response to low serum calcium and induces the production of calcitriol. In contrast, reduced levels of PTH stimulate synthesis of the inactive 24,25-(OH)2D3. In the intestinal epithelium, calcitriol functions as a steroid hormone in inducing the expression of calbindinD28K, a protein involved in intestinal calcium absorption. The steroid hormone action of vitamin D occurs via the action of calcitriol binding to a specific intracellular receptor that is a member of the nuclear receptor family of hormone receptors called the vitamin D receptor (VDR).

The increased absorption of calcium ions requires concomitant absorption of a negatively charged counter ion to maintain electrical neutrality. The predominant counter ion is Pi. When plasma calcium levels fall the major sites of action of calcitriol and PTH are bone where they stimulate bone resorption and the kidneys where they inhibit calcium excretion by stimulating reabsorption by the distal tubules. The role of calcitonin in calcium homeostasis is to decrease elevated serum calcium levels by inhibiting bone resorption.

Due to the critical role of Vitamin D in the development of growing bone as well as the maintenance of healthy bone it has been added to milk since the 1930's. In the US all milk is fortified with 100 IU/cup. Fatty fishes such as salmon, sardines and tuna are also an excellent source of vitamin D. Cod liver oil is also very high in vitamin D. For vegetarians and vegans ultraviolet (uv) light irradiated mushrooms and yeast are the only sources of vitamin D that are not animal in origin. The irradiation of yeasts and mushrooms produces vitamin D2. Vitamin D content is listed as the amount in International Units (IU).


Food source

Vitamin D content (IU)

Cod liver oil, 1Tbs (15ml) 1360
Salmon, cooked, 3.5 oz 447
Sardines, canned in oil, 1.75 oz 250
Tuna, canned in water, 3 oz 154
Egg, 1 large 41
Swiss cheese 6
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Clinical Significances of Vitamin D Deficiency

As a result of the addition of vitamin D to milk, deficiencies in this vitamin are rare in this country. The main symptom of vitamin D deficiency in children is rickets and in adults is osteomalacia. Rickets is characterized improper mineralization during the development of the bones resulting in soft bones. Osteomalacia is characterized by demineralization of previously formed bone leading to increased softness and susceptibility to fracture.

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Vitamin E

Structure of the tocopherols

Structures of the 4 tocopherols

Vitamin E is a mixture of several related compounds known as tocopherols and tocotrienols. The tocopherols are the major sources of vitamin E in the U.S. diet. The tocopherols differ by the number and position of methyl (–CH3–) groups present on the ring system of the chemical structure. The different tocopherols are designated α-, β-, γ-, and δ-tocopherol. Most vitamin E in U.S. diets is in the form of γ-tocopherol from soybean, canola, corn, and other vegetable oils. All four tocopherols are able to act as free radical scavengers thus they all have potent antioxidant properties. Vitamin E is absorbed from the intestines packaged in chylomicrons. It is delivered to the tissues via chylomicron transport and then to the liver through chylomicron remnant uptake. The liver can export vitamin E in very low density lipoproteins (VLDLs). Within the liver α-tocopherol transfer protein preferentially transfers α-tocopherol to VLDLs, thus α-tocopherol is the most abundant tocopherol in nonhepatic (liver) tissues. Due to its lipophilic nature, vitamin E accumulates in cellular membranes, fat deposits and other circulating lipoproteins. The major site of vitamin E storage is in adipose tissue.

The major function of vitamin E is to act as a natural antioxidant by scavenging free radicals and molecular oxygen. In particular vitamin E is important for preventing peroxidation of polyunsaturated membrane fatty acids. The vitamins E and C are interrelated in their antioxidant capabilities. Active α-tocopherol can be regenerated by interaction with vitamin C following scavenge of a peroxy free radical. Alternatively, α-tocopherol can scavenge two peroxy free radicals and then be conjugated to glucuronate for excretion in the bile.

Although α-tocopherol is the most abundant tocopherol in tissues outside of the liver, it is not the most potent antioxidant form of the vitamin. Because of the unmethylated carbons in the ring structure of γ- and δ-tocopherol, these two forms of vitamin E are much more active at trapping free radicals, in particular reactive nitrogen species. In addition, research has recently shown that the anticancer effects of vitamin E are due to the γ- and δ-tocopherol forms and is not associated with α-tocopherol.

Recommended daily intake amounts for vitamin E (as α-tocopherol) are listed in milligram (mg) amounts and also in International Units (IU). To convert from mg to IU: 1 mg of α-tocopherol is equivalent to 1.49 IU of the natural form or 2.22 IU of the synthetic form. Nuts, seeds, and vegetable oils are among the best sources of α-tocopherol, and significant amounts are available in green leafy vegetables and fortified cereals. Most vitamin E in US diets is in the form of γ-tocopherol from soybean, canola, corn, and other vegetable oils.


Food source

Vitamin E content (mg)

Wheat germ oil, 1 tablespoon 20.3
Almonds, dry roasted, 1 ounce 7.4
Sunflower oil, 1 tablespoon 5.6
Safflower oil, 1 tablespoon 4.6
Peanut butter, 2 tablespoons 2.9
Peanuts, dry roasted, 1 ounce 2.2
Corn oil, 1 tablespoon 1.9
Broccoli, chopped, boiled, ½ cup 1.2
Soybean oil, 1 tablespoon 1.1
Kiwifruit, 1 medium 1.1
Spinach, raw, 1 cup 0.6
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Clinical Significances of Vitamin E Deficiency

No major disease states have been found to be associated with vitamin E deficiency due to adequate levels in the average American diet. The major symptom of vitamin E deficiency in humans is an increase in red blood cell fragility. Since vitamin E is absorbed from the intestines in chylomicrons, any fat malabsorption diseases can lead to deficiencies in vitamin E intake. Neurological disorders have been associated with vitamin E deficiencies associated with fat malabsorptive disorders. Increased intake of vitamin E is recommended in premature infants fed formulas that are low in the vitamin as well as in persons consuming a diet high in polyunsaturated fatty acids. Polyunsaturated fatty acids tend to form free radicals upon exposure to oxygen and this may lead to an increased risk of certain cancers.

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Vitamin K

The K vitamins exist naturally as K1 (phylloquinone) in green vegetables and K2 (menaquinone) produced by intestinal bacteria and K3 is synthetic menadione. When administered, vitamin K3 is alkylated to one of the vitamin K2 forms of menaquinone.

Structure of vitamin K1

Vitamin K1

 
Structure of vitamin K2

"n" can be 6, 7 or 9 isoprenoid groups

Structure of vitamin K3

Vitamin K2

Vitamin K3

The major function of the K vitamins is in the maintenance of normal levels of the blood clotting proteins, factors II, VII, IX, X and protein C and protein S, which are synthesized in the liver as inactive precursor proteins. Conversion from inactive to active clotting factor requires a posttranslational modification of specific glutamate (E) residues. This modification is a carboxylation and the enzyme responsible requires vitamin K as a cofactor. The resultant modified E residues are γ-carboxyglutamate (gla). This process is most clearly understood for factor II, also called preprothrombin. Prothrombin is modified preprothrombin. The gla residues are effective calcium ion chelators. Upon chelation of calcium, prothrombin interacts with phospholipids in membranes and is proteolysed to thrombin through the action of activated factor X (Xa).

During the carboxylation reaction reduced hydroquinone form of vitamin K is converted to a 2,3-epoxide form. The regeneration of the hydroquinone form requires the action of the enzyme vitamin K epoxide reductase (VKORC1) which involves a two-step reaction. These latter reactions are the site of action of the coumarin based anticoagulants such as warfarin (trade name = Coumadin®).

Structure of a γ-carboxyglutamamte (gla) residue and mechanism of incorporation into protein

Incorporation of a gla-residue into prothrombin: The incorporation of a gla-residue into a protein such as prothrombin requires the hydroquinone (KH2) form of vitamin K (either K1, K2, or synthetic K3). The utilization and regeneration of the KH2 form in the overall process of the γ-glutamyl carboxylase (GGCX) reaction is referred to as the vitamin K cycle. Either following the carboxylation, or directly from dietary quinone forms of vitamin K, the action of vitamin K epoxide reductase (VKORC1) is to provide a continuous source of the KH2 form. An addition enzyme, vitamin K quinone reductase (VKQR) can catalyze the conversion of the quinone form to the hydroquinone form using NADH as a cofactor.

In the average U.S. diet, meats and eggs are the most common food sources of the menaquinone form of vitamin K. Excellent sources of vitamin K include spinach, Brussels sprouts, Swiss chard, green beans, asparagus, broccoli, kale, mustard greens, green peas and carrots. Fermentation of foods can increase their vitamin K content. Fermented soy foods play a unique role in supplying vitamin K in certain traditional cuisines (like that of Japan). Sometimes you will see the word "natto" being used to refer to these fermented soy foods since Bacillus natto are bacteria that can convert vitamin K1 into K2 and are often used in the production of fermented soy products. Some cheeses are also fermented in a way that optimizes their vitamin K content.

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Clinical Significances of Vitamin K Deficiency

Naturally occurring vitamin K is absorbed from the intestines only in the presence of bile salts and other lipids through interaction with chylomicrons. Therefore, fat malabsorptive diseases can result in vitamin K deficiency. The synthetic vitamin K3 is water soluble and absorbed irrespective of the presence of intestinal lipids and bile. Since the vitamin K2 form is synthesized by intestinal bacteria, deficiency of the vitamin in adults is rare. However, long term antibiotic treatment can lead to deficiency in adults. The intestine of newborn infants is sterile, therefore, vitamin K deficiency in infants is possible if lacking from the early diet. The primary symptom of a deficiency in infants is a hemorrhagic syndrome.

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Alpha-Lipoic Acid, LA

Alpha-lipoic acid, LA, (chemical name: 1,2-dithiolane-3-pentanoic acid; also known as thioctic acid), is a naturally occurring dithiol compound synthesized enzymatically in the mitochondrion from the medium-chain fatty acid octanoic acid. Because LA can be synthesized in the body it is not technically considered a vitamin but because of its vital role in overall cellular metabolism it is considered as an important, but not necessary,  dietary supplement. Lipoic acid has one chiral center and therefore exists in both R- and S-enantiomeric forms (see the Figure below). However, only R-LA is conjugated to conserved lysine residues in an amide linkage (forming a lipoamide), thus making this isoform essential as a cofactor in biological systems. Enzymes containing lipoamide are typically mitochondrial multi-enzyme complexes that catalyze the oxidative decarboxylation of α-keto acids (e.g. pyruvate dehydrogenase, α-ketoglutarate dehydrogenase, and transketolase) and glycine cleavage. The TCA cycle enzyme, α-ketoglutarate dehydrogenase, is also known as 2-oxoglutarate dehydrogenase.

LA is a necessary cofactor for mitochondrial α-ketoacid dehydrogenases, and thus serves a critical role in mitochondrial energy metabolism. In addition to de novo synthesis, LA is also absorbed intact from dietary sources, and it transiently accumulates in many tissues. LA has been described as a potent biological antioxidant, a detoxification agent, and a diabetes medicine; it has been used to improve age-associated cardiovascular, cognitive, and neuromuscular deficits, and has been implicated as a modulator of various inflammatory signaling pathways. Accumulating evidence indicates that LA supplied as a supplement in the diet may not be used as a metabolic cofactor but instead, elicits a unique set of biochemical activities with potential therapeutic value against a host of pathophysiological insults.


structure of alpha-lipoic acid

Structure of α-Lipoic Acid


Lipoic acid, either as a dietary supplement or a therapeutic agent, modulates distinct red-ox circuits because of its ability to equilibrate between different subcellular compartments as well as extracellularly. Because of its role in regulating redox states LA is a critical component of the antioxidant network. It is important in the regeneration of other antioxidants, such as vitamins E and C, it increases the intracellular levels of glutathione (GSH), and provides redox regulation of numerous proteins and transcription factors. The extracellular thiol/disulfide red-ox environment (determined by the interconversion between cysteine and cystine) is able to modulate cell proliferation, apoptosis, cell adhesion molecules, and proinflammatory signaling. Lipoic acid may play an important role in the modulation of the extracellular red-ox state via the involvement of dihydrolipoic acid (DHLA) in the reduction of cystine to cysteine. This reduction facilitates a rapid uptake of the cysteine into the cell making it available to stimulate GSH synthesis. Cellular uptake of LA has been shown to occur via several systems including the medium chain fatty acid transporter, a Na+-dependent vitamin transport system, and a H+-linked monocarboxylate transporter for intestinal uptake. The cellular reduction of LA to DHLA is accomplished by NAD(P)H-driven enzymes, thioredoxin reductase, lipoamide dehydrogenase, and glutathione reductase.


interconversions of dihydrolipoic acid (DHLA) and lipoic acid (LA)

Interconversions of α-lipoic acid (LA) and dihydrolipoic acid (DHLA)


Lipoic acid is an essential cofactor for the E2 component of α-ketoacid dehydrogenase complexes, exclusively located in mitochondria. These include the pyruvate dehydrogenase (PDH), α-ketoglutarate dehydrogenase (KGDH), and branched chain α-ketoacid dehydrogenase (BCKDH) complexes. PDH catalyzes the oxidative carboxylation of pyruvate which serves as the entry point for carbohydrates into the TCA cycle as acetyl-CoA. KGDH serves as a regulatory enzyme in the TCA cycle. Evidence demonstrates that the activities of both PDH and KGDH are substantially decreased during aging and in neurodegenerative disorders.

Extensive evidence suggests that LA may have therapeutic usefulness in lowering blood glucose levels in diabetic conditions and that the intracellular redox status plays a role in the modulation of insulin resistance. Lipoic acid has been shown to stimulate glucose uptake by affecting components of the insulin signaling pathway. The signaling networks of insulin receptor activation include the insulin receptor substrates (IRS1, IRS2, IRS3 and IRS4), PI3K, and AKT/PKB. Insulin-mediated activation of PI3K and AKT/PKB is necessary for the translocation of GLUT4 from an intracellular pool to the plasma membrane to allow for uptake of plasma glucose. Lipoic acid has been shown to augment tyrosine phosphorylation and the activity of components of insulin signaling including the insulin receptor, IRS1, PI3K, AKT1, and p38MAPK. In addition, LA stimulated glucose uptake upon translocation and regulation of the intrinsic activity of GLUT4, an effect that is likely mediated by p38 MAPK. R-LA, as well as oxidized isoforms have been shown to stimulate glucose transport in differentiated 3T3-L1 adipocytes by a mechanism entailing changes in the intracellular redox status. These effects of LA are in agreement with an alteration of the thiol reactivity of redox components of the insulin signaling pathway caused by a thiol/disulfide exchange mechanism.

The stress-activated MAPK and JNK pathways play a central role in the progression of insulin resistance and diabetic neuropathies. The activation of these kinases results in the phosphorylation of IRS1 on serine 307 resulting in inhibition of the insulin-stimulated tyrosine phosphorylation of IRS1 leading to inhibition of insulin signaling. Lipoic acid has been shown to inhibit the JNK pathway and IRS1 serine phosphorylation, resulting in improved insulin sensitivity. The precise mechanism by which LA inhibits the JNK pathway remains unclear. Nevertheless, these effects place LA at the cross-road of insulin- and JNK signaling favoring glucose uptake and metabolism, thus ameliorating insulin resistance and improving the status of diabetes.

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Minerals

The functions of the "minerals" are numerous and either quite broad or highly specific. The following list of minerals and their functions is not intended to be exhaustive.

Calcium is required for bone mineralization, cardiac function, muscle contraction, and digestive system function. In addition, calcium is necessary for proper activity of a number of proteins involved in blood coagulation.

Chlorine (as chloride ion) is important to maintain the function of numerous cellular pumps and is used in the production of hydrochloric acid (HCl) in the stomach.

Iron, although considered a trace element, has a critical role in the transport of oxygen. Iron is the functional center of the heme moiety found in each of the protein subunit of hemoglobin. The function of iron is to coordinate the oxygen molecule into heme of hemoglobin so that it can be transported from the lungs to the tissues.

Magnesium is required for bone mineralization as well as for the proper functioning of ATP. In this latter function, essentially all of ATP in the cell is has magnesium bound to the phosphates. This magnesium:ATP complex allows ATP to more readily release the terminal phosphate (the γ-phosphate) when doing so to provide energy for cellular metabolism.

Phosphorous is the most important systemic electrolyte acting as a significant buffer in the blood in the form of phosphate ion: PO42–. Phosphate is also required for bone mineralization, and is necessary for energy utilization.

Potassium is a key circulating electrolyte as well as being involved in the regulation of ATP-dependent channels along with sodium. These channels are referred to as Na+/K+-ATPases and their primary function is in the transmission of nerve impulses in the brain.

Sodium is a key circulating electrolyte and also functions in the regulation of ATP-dependent channels with potassium. These channels are referred to as Na+/K+-ATPases and their primary function is in the transmission of nerve impulses in the brain.

Sulfur has a primary function in amino acid metabolism but is also necessary for the modification of complex carbohydrates present in proteins and lipids, however, it should be noted that in this latter function the sulfur comes from the amino acid methionine.

The trace minerals function primarily as cofactors or regulators of enzyme function The terminology of "trace" relates to the fact that these minerals are effective and necessary in only minute concentration.

Copper is involved in the formation of red bloods cells, the synthesis of hemoglobin, and the formation of bone. Additional functions of copper are energy production, wound healing, taste sensation, skin and hair color. Copper is also involved in the proper processing of collagen (the most abundant protein in the body) and thus, is important in skin, bone, and connective tissue production.

Iodine is required for the synthesis of the thyroid hormones and thus plays an important role in the regulation of energy metabolism via thyroid hormone functions.

Manganese is involved in reactions of protein and fat metabolism, promotes a healthy nervous system, necessary for digestive function, bone growth, and immune function. In addition, manganese is necessary for the proper function of super oxide dismutase (SOD) which is an enzyme required for preventing super oxide anions from damaging cells.

Molybdenum is primarily involved as a co-factor in several oxidases such as xanthine oxidase (purine nucleotide catabolism), aldehyde oxidase, and sulfite oxidase.

Selenium serves as a modifier of the activity of glutathione peroxidase through its incorporation into the protein in the form of selenocysteine. The role of selenocysteine in protein synthesis is described in the Protein Synthesis page.

Zinc is found as a co-factor in over 300 different enzymes and thus is involved in a wide variety of biochemical processes. Zinc interacts with the hormone insulin to ensure proper function and thus this trace mineral has an important role in regulation of blood glucose levels via insulin action. Zinc also promotes wound healing, regulates immune function, serves as a co-factor for numerous antioxidant enzymes, and is necessary for protein synthesis and the processing of collagen.

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RDA Values for Vitamins and Minerals

Click the following link to open a PDF file containing the recommended daily intake values for various vitamins and minerals:

RDA Tables for Vitamins and Minerals

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Last modified: July 11, 2014