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Michael W. King 
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Molecular and Developmental Biology
Studies of Gene Function in Regeneration, Early Development and Cancer
Executive Member, IU Center for Regenerative Biology and Medicine
Professor of Biochemistry and Molecular Biology, IU School Medicine
Professor of Biology, Indiana State University
Research Professor of Applied Biology and Biomedical Engineering, Rose-Hulman Institute of Technology
Ph.D. Biochemistry, University of California at Riverside, 1984
Research being conducted in my laboratory centers on the isolation and characterization of novel factors involved in tissue regeneration. Frogs represent a useful animal model with which to study molecular mechanisms that drive regeneration and, conversely, which repress gene activity that could lead to inhibition of the regenerative capacity in higher vertebrates. In these species limbs and spinal cords regenerate well during larval stages, but gradually lose this ability as the animal approaches metamorphosis. Adult frogs do not regenerate and the response of these structures to surgical transection is normally similar to that of higher vertebrates. This stage difference in regenerative ability can be used to advantage experimentally to discover, by differential gene screening, the molecules and molecular pathways that drive regeneration or inhibit regeneration within the same species. This project is being carried out with a consortium of researchers at Indiana University, Bloomington, IUPUI Indianapolis and Eli Lilly and Co., Indianapolis
Treatment of axlotl limbs (which can regenerate throughout the life of this urodele) with beryllium sulfate after amputation abolishes the ability of the limbs to regenerate. It is known that beryllium augments inflammatory responses and thus, its ability to interfere with regeneration may be related to this immune system response. We treated stage 53 Xenopus laevis hindlimbs (regeneration capable stage) with beryllium sulfate immediately after amputation and then collected limb blastema tissue samples at various times for RNA extraction and RT-PCR analysis of gene expression levels. We examined the expression of genes involved in the inflammatory response, tissue reprogramming and pluripotency as well as genes involved in patterning the blastema during regeneration. These data show that beryllium does indeed increase the immune gene expression while simultaneously inhibiting blastema patterning with little or no effect on reprogramming gene expression.
Selected Publications from Last Five Years
Research
Wilson, JM, Martinez-De Luna,RI, El Hodiri, HM, Smith, R, King, MW, Mescher, AL, Neff, AW and Belecky-Adams, TL. 2010 RNA helicase Ddx39 is expressed in the developing central nervous system, limb, otic vesicle, branchial arches and facial mesenchyme of Xenopus laevis Gene Expression Patterns 10(1):44-52
Rao, N, Jhamb, D, Milner, DJ, Li, B, Song, F, Wang, M, Voss, SR, Palakal, M, King, MW, Saranjami, B, Nye, HLD, Cameron, JA, and Stocum, DL 2009 Proteomic analysis of blastema formation in regenerating axolotl limbs. BMC Biology 7:83
Malloch, E, Perry, KJ, Fukui, L, Johnson, V, Cheng, M, Wever, J, Beck, CW, King, MW, and Henry, JJ. 2009 Gene Expression Profiles of Lens Regeneration and Development in Xenopus laevis. Developmental Dynamics 238:2340-2356
King, MW, Neff, AW and Mescher, AL 2009 Proteomics analysis of regenerating amphibian limbs: changes during the onset of regeneration. International Journal of Developmental Biology 53(7):955-969
Million Passe, CM, White, CR, King, MW, Quirk, PL, Iovanna, JL and Quirk, CC 2008 Loss of the protein NUPR1 (p8) leads to delayed LHb expression, delayed ovarian maturation, and testicular development of a Sertoli-cell-only syndrome-like phenotype. Biology of Reproduction 79:598-607
Werner, SR, Mescher, AL, Neff, AW, King, MW, Harty, MW, Smith, RC. 2007 Neural MMP-28 expression precedes myelination during development and peripheral nerve repair. Developmental Dynamics 236:2852-2864
Brannon, KM, Million Passe, CM, White, CR, A Bade, N., King, MW, Quirk, CC 2007 Expression of the high mobility group A family member p8 is essential to maintaining tumorigenic potential by promoting cell cycle dysregulation in LβT2 cells. Cancer Letters 254:146-155
Alshaibi, N, King, MW, Duong, T, and Ghosh, SK 2007 DP58, an inducible myeloid protein, is constitutively expressed in murine neuronal nuclei. Frontiers of Bioscience 12:2947-2956
Grow, MW, Neff, AW, Mescher, AL and King, MW 2006 Global analysis of gene expression in Xenopus hindlimbs during stage-dependent complete and incomplete regeneration. Developmental Dynamics 235:2667-2685
Neff, A.W., King, MW, Harty, M.W., Nguyen, T., Calley, J., Smith, R.C. and Mescher, A.L. 2005 Expression of Xenopus XlSALL4 during limb development and regeneration. Developmental Dynamics 233: 356-367
Education Publications
Textbook Editor:
Lange Q&A USMLE Step 1, 6th edition, 2008. McGraw-Hill, NY
Lange Q&A USMLE Step 1, 5th edition, 2005. McGraw-Hill, NY
Appleton & Lange’s Review for the USMLE Step 1, 4th edition, 2002. McGraw-Hill, NY
Textbooks:
Biochemistry: Examination and Board Review, Balcavage, WX and King, MW 1995. Appleton and Lange, Stamford, CT.
Textbook Book Chapters in Last Three Years:
King, MW 2008 2008 Chapter 3: Biochemistry, and Practice Tests (Chapter 8 - 14) in: Lange Q&A USMLE Step I, 6th ed. McGraw-Hill, NY, NY.
King, MW 2007 Chapter 7, pp 177-207: Genetic Mechanisms in Cell and Molecular Biology for Engineers, ed. Waite and Waite, McGraw-Hill, NY, NY.
King, MW 2007 Chapter 9, pp 233-253: Cellular Development in Cell and Molecular Biology for Engineers, ed. Waite and Waite, McGraw-Hill, NY, NY.
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Created by: Michael W. King, Ph.D. Last modified: February 22, 2010 |
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miking at iupui.edu |