Michael W. King

miking @ iupui.edu

Molecular and Developmental Biology

Studies of Gene Function in Regeneration, Early Development and Cancer

Executive Member IU Center for Regenerative Biology and Medicine
Professor of Biochemistry and Molecular Biology, IU School Medicine
Professor of Life Sciences, Indiana State University
Research Professor of Applied Biology and Biomedical Engineering, Rose-Hulman Institute of Technology

Ph.D. Biochemistry, University of California at Riverside, 1984

Research being conducted in my laboratory centers on the isolation and characterization of novel factors involved in tissue regeneration. Frogs represent a useful animal model with which to study molecular mechanisms that drive regeneration and, conversely, which repress gene activity that could lead to inhibition of the regenerative capacity in higher vertebrates. In these species limbs and spinal cords regenerate well during larval stages, but gradually lose this ability as the animal approaches metamorphosis. Adult frogs do not regenerate and the response of these structures to surgical transection is normally similar to that of higher vertebrates. This stage difference in regenerative ability can be used to advantage experimentally to discover, by differential gene screening, the molecules and molecular pathways that drive regeneration or inhibit regeneration within the same species. This project is being carried out with a consortium of researchers at Indiana University, Bloomington, IUPUI Indianapolis and Eli Lilly and Co., Indianapolis

Expression of Immune and Patterning Genes During Limb Regeneration

Expression levels of a select set of genes found in a screen of the Affymetrix Xenopus genechip were assayed by RT-PCR and qPCR to validate the array results. RT-PCRs were carried out for 25 cycles except for those genes identified with an asterisk (30 cycles). Gene names or symbols are indicated to the left of each panel. Numbers below each panel represent the qPCR values for expression level when normalized to ODC and with 1dPA samples arbitrarily set to a value of 1. Tables to the right of each panel compare the ratios of gene expression determined by qPCR with those determined from the array screen. Columns A, B and C refer to the designations outlined in Figure 1 and the number coloring is the same as described in Grow et al., 2006, e.g. green numbers for panel A indicate that the expression ratio is higher in st53 1dPA compared to st57 1dPA. A dash indicates that there was no statistically significant ratio for that value from the array screen. ODC = ornithine decarboxylase, SOCS3 = suppressor of cytokine signaling 3, FGL2 = fibrinogen-like protein 2, C3 = complement factor 3, nNOS = neuronal nitric oxide synthase

Selected Publications from Last Five Years

Research


Werner, SR, Mescher, AL, Neff, AW, King, MW, Harty, MW, Smith, RC. 2007 Neural MMP-28 expression precedes myelination during development and peripheral nerve repair. Developmental Dynamics 236:2852-2864

Brannon, KM, Million Passe, CM, White, CR, A Bade, N., King, MW, Quirk, CC 2007 Expression of the high mobility group A family member p8 is essential to maintaining tumorigenic potential by promoting cell cycle dysregulation in LbT2 cells. Cancer Letters 254:146-155

Alshaibi, N, King, MW, Duong, T, and Ghosh, SK 2007 DP58, an inducible myeloid protein, is constitutively expressed in murine neuronal nuclei. Frontiers of Bioscience 12:2947-2956

Grow, MW, Neff, AW, Mescher, AL and King, MW 2006 Global analysis of gene expression in Xenopus hindlimbs during stage-dependent complete and incomplete regeneration. Developmental Dynamics 235:2667-2685

Neff, A.W., King, MW, Harty, M.W., Nguyen, T., Calley, J., Smith, R.C. and Mescher, A.L. 2005 Expression of Xenopus XlSALL4 during limb development and regeneration. Developmental Dynamics 233: 356-367

Harty, M., Neff, A.W., King, M.W., and Mescher, A.L. 2003. Regeneration or Scarring: An Immunological Perspective. Developmental Dynamics 226: 268-279

King, M.W., Nguyen, T., Calley, J., Harty, M.W., Muzinich, M.C., Mescher, A.L., Chalfant, C., N'Cho, M., McLeaster, K., McEntire, J., Stocum, D., Smith, R.C., Neff, A.W. 2003. Identification of genes expressed during Xenopus laevis limb regeneration using subtractive hybridization. Developmental Dynamics 226: 398-409

Education Publications

Textbook Editor

Lange Q&A USMLE Step 1, 6th edition, 2008. McGraw-Hill, NY

Lange Q&A USMLE Step 1, 5th edition, 2005. McGraw-Hill, NY

Appleton & Lange’s Review for the USMLE Step 1, 4th edition, 2002. McGraw-Hill, NY

Textbooks

Biochemistry: Examination and Board Review, Balcavage, WX and King, MW 1995. Appleton and Lange, Stamford, CT.

Textbook Book Chapters in Last Two Years

King, MW 2008 2008 Chapter 3: Biochemistry, and Practice Tests (Chapter 8 - 14) in: Lange Q&A USMLE Step I, 6th ed. McGraw-Hill, NY, NY.

King, MW 2007 Chapter 7, pp 177-207: Genetic Mechanisms in Cell and Molecular Biology for Engineers, ed. Waite and Waite, McGraw-Hill, NY, NY.

King, MW 2007 Chapter 9, pp 233-253: Cellular Development in Cell and Molecular Biology for Engineers, ed. Waite and Waite, McGraw-Hill, NY, NY.

Created by: Michael W. King, Ph.D.
Last modified: April 10, 2008 		return to The Medical Biochemistry Page

miking at iupui.edu